Journal: Applied Sciences

Article Title: Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap

doi: 10.3390/app14062340

Figure Lengend Snippet: Figure 3. Bar graph showing the mean CFU obtained in SDA prepared with conventional sterilization (control, in an autoclave (or by boiling)), and in a microwave oven for 7 min (7′), 10 min (10′), 13 min (13′), and 15 min (15′), after inoculation using the pour plate method, for C. albicans, S. cerevisiae, and A. brasiliensis.

Article Snippet: Culture Media Assay Micro-Organism ATCC 1 WDCM 2 TSA Productivity assays Escherichia coli 8739 00012 Candida albicans 10231 00054 Pseudomonas aeruginosa 9027 00026 Staphylococcus aureus 6538 00032 Aspergillus brasiliensis 16404 00053 Bacillus subtilis 6633 00003 Listeria monocytogenes 13932 00021 Salmonella enterica subsp. enterica ser.

Techniques: Control

Journal: Applied Sciences

Article Title: Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap

doi: 10.3390/app14062340

Figure Lengend Snippet: Figure 4. Bar graph showing the mean CFU obtained in TSA prepared with conventional sterilization (control, in an autoclave (or by boiling)), and in a microwave oven for 7 min (7′), 10 min (10′), 13 min (13′), and 15 min (15′), after inoculation using the pour plate method, for E. coli, C. albicans, S. aureus, B. subtilis, L. monocytogenes, A. brasiliensis, P. aeruginosa, S. ser. Typhimurium, and C. perfringens.

Article Snippet: Culture Media Assay Micro-Organism ATCC 1 WDCM 2 TSA Productivity assays Escherichia coli 8739 00012 Candida albicans 10231 00054 Pseudomonas aeruginosa 9027 00026 Staphylococcus aureus 6538 00032 Aspergillus brasiliensis 16404 00053 Bacillus subtilis 6633 00003 Listeria monocytogenes 13932 00021 Salmonella enterica subsp. enterica ser.

Techniques: Control

Journal: Molecules (Basel, Switzerland)

Article Title: Poly-(lactic- co -glycolic) Acid Nanoparticles Entrapping Pterostilbene for Targeting Aspergillus Section Nigri .

doi: 10.3390/molecules27175424

Figure Lengend Snippet: Figure 2. Overlap of the bright field image and the fluorescence image, which shows A. brasiliensis conidia treated for 10 min with 50 nm PLGA-coumarin6-NPs. In the first stage of conidia development (yellow arrow) the protective envelope did not allow interaction with NPs. In a later stage of conidia development (red arrow), when the envelope broke, fluorescence along the conidia capsule was observed (A). A 3D reconstruction of A. brasiliensis conidia treated with NPs for 10 min. The fluorescence signal was detected along the wall of the conidia (B). Fluorescence image of the hyphae of the newly germinated A. brasiliensis conidium treated with 50 nm PLGA-coumarin6 -NPs. The fluorescence signal inside A. brasiliensis hyphae is visible after 1 h of NPs administration (C).

Article Snippet: A. brasiliensis (DSM 1988), formerly A. niger (DSM 1988), species Aspergillus section Nigri from the German Collection of Microorganisms (DSMZ, Braunschweig, Germany) was used as a reference strain in this study.

Techniques: Fluorescence

Journal: Molecules (Basel, Switzerland)

Article Title: Poly-(lactic- co -glycolic) Acid Nanoparticles Entrapping Pterostilbene for Targeting Aspergillus Section Nigri .

doi: 10.3390/molecules27175424

Figure Lengend Snippet: Figure 3. Observation of A. brasiliensis mycelium and biofilm treated with 50 nm PLGA-coumarin6- NPs. Overlap of the bright field image and the fluorescence image, which shows the localization of PLGA-coumarin6-NPs inside the fungal hypha (A). Presence of NPs within the biofilm (B). A 3D reconstruction of biofilm treated with NPs for 60 min. The fluorescence signal was detected along the entire thickness of the biofilm matrix (C).

Article Snippet: A. brasiliensis (DSM 1988), formerly A. niger (DSM 1988), species Aspergillus section Nigri from the German Collection of Microorganisms (DSMZ, Braunschweig, Germany) was used as a reference strain in this study.

Techniques:

Journal: Molecules (Basel, Switzerland)

Article Title: Poly-(lactic- co -glycolic) Acid Nanoparticles Entrapping Pterostilbene for Targeting Aspergillus Section Nigri .

doi: 10.3390/molecules27175424

Figure Lengend Snippet: Figure 4. Activity of free PTB and PLGA-PTB-NPs against A. brasiliensis (DSM 1988). Activity of free PTB and PLGA-PTB-NPs against A. brasiliensis biofilm in formation, after 24 h of incubation (A). Activity of free PTB and PLGA-PTB-NPs against A. brasiliensis 24 h biofilm, after 24 h of incubation (B). Activity of free PTB and PLGA-PTB-NPs against 48 h biofilm, after 24 h of incubation (C). * p < 0.05 compared to the control; ** p < 0.01 compared to the control *** p < 0.001 compared to the control.

Article Snippet: A. brasiliensis (DSM 1988), formerly A. niger (DSM 1988), species Aspergillus section Nigri from the German Collection of Microorganisms (DSMZ, Braunschweig, Germany) was used as a reference strain in this study.

Techniques: Activity Assay, Incubation, Control

Journal: Molecules (Basel, Switzerland)

Article Title: Poly-(lactic- co -glycolic) Acid Nanoparticles Entrapping Pterostilbene for Targeting Aspergillus Section Nigri .

doi: 10.3390/molecules27175424

Figure Lengend Snippet: Figure 5. PLGA-PTB-NPs reduces A. brasiliensis virulence in G. mellonella model. Survival curves of G. mellonella larvae (n = 10/strain) infected via injection with 2 × 104 conidia from A. brasiliensis with free PTB. Larvae were monitored for 5 days post-infection. Statistical significance relative to control was judged by the Kaplan–Meier followed by Mantel–Cox log-rank tests. At least three independent biological replicates were carried out for each experiment. * p < 0.05 compared to the free PTB and PLGA-PTB-NPs; ** p < 0.01 compared to the free PTB and PLGA-PTB-NPs.

Article Snippet: A. brasiliensis (DSM 1988), formerly A. niger (DSM 1988), species Aspergillus section Nigri from the German Collection of Microorganisms (DSMZ, Braunschweig, Germany) was used as a reference strain in this study.

Techniques: Infection, Injection, Control

Journal: bioRxiv

Article Title: Global survey of secondary metabolism in Aspergillus niger via activation of specific transcription factors

doi: 10.1101/2024.07.18.604165

Figure Lengend Snippet: A) Schematic representation of the NRRL3_04231 BGC. B) High degree of synteny exists between A. niger, A. brasiliensis, and A. tubingensis at the site of this BGC, which is absent in other Aspergilli identified as non-producers of tensidols. C) Chemical structure of tensidol B. D) Extracted ion chromatograms (EIC) of tensidol B production found in culture extract from A. brasiliensis , NRRL3_04231 overexpression strain, and the parental strain NRRL2270. The peak intensity for each biological replicate is shown inset in the respective EIC panels. n.d. – not detected.

Article Snippet: To further assess the ability of the NRRL3_04231 OE strain to produce tensidol B, we obtained a known producer of tensidol B, A. brasiliensis strain SN26 (ATCC 9642).

Techniques: Over Expression